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KMID : 0880220120500040684
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Journal of Microbiology
2012 Volume.50 No. 4 p.684 ~ p.688
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Evaluation of a fosmid-clone-based microarray for comparative analysis of swine fecal metagenomes
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Park Soo-Je
Jung Man-Young
Kim So-Jeong
Kim Yang-Hoon
Rhee Sung-Keun
Chae Jong-Chan
Kim Hong-Ik
Kim Dong-Hwan
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Abstract
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Glass slide arrayed with fosmid clone DNAs generated from swine feces as probes were fabricated and used as a metagenome microarray (MGA). MGA appeared to be specific to their corresponding target genomic fragments. The detection limit was 10 ng of genomic DNA (ca. 106 bacterial cells) in the presence of 1000 ng of background DNA. Linear relationships between the signal intensity and the target DNA (20?100 ng) were observed (r2=0.98). Application of MGA to the comparison of swine fecal metagenomes suggested that the microbial community composition of swine intestine could be dependent on the health state of swine.
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KEYWORD
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microarray, fosmid clone, gut, community analysis
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